Amphoteric substance has both acid and base qualities. Ampholytes are amphoteric molecules that contain both acidic and basic groups and will exist mostly as zwitterions in a certain range of pH. The pH at which the average charge is zero is known as the molecule’s isoelectric point. Ampholytes are used to establish a stable pH gradient for use in isoelectric focusing.

March 19, 2011 | Imran Jutt | No Comments | 513 views